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	<title>生信菜鸟团 &#187; workflow</title>
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		<title>自学CHIP-seq分析第二讲~学习资料的搜集</title>
		<link>http://www.bio-info-trainee.com/1736.html</link>
		<comments>http://www.bio-info-trainee.com/1736.html#comments</comments>
		<pubDate>Tue, 05 Jul 2016 00:20:00 +0000</pubDate>
		<dc:creator><![CDATA[ulwvfje]]></dc:creator>
				<category><![CDATA[tutorial]]></category>
		<category><![CDATA[CHIP-seq]]></category>
		<category><![CDATA[Motif]]></category>
		<category><![CDATA[Peak]]></category>
		<category><![CDATA[visualization]]></category>
		<category><![CDATA[workflow]]></category>

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		<description><![CDATA[我只能说，CHIP-seq的确是非常完善的NGS流程了，各种资料层出不穷，大家首 &#8230; <a href="http://www.bio-info-trainee.com/1736.html">Continue reading <span class="meta-nav">&#8594;</span></a>]]></description>
				<content:encoded><![CDATA[<div>我只能说，CHIP-seq的确是非常完善的NGS流程了，各种资料层出不穷，大家首先可以看下面几个完整流程的PPT来对CHIP-seq流程有个大致的印象，我对前面提到的文献数据处理的几个要点，就跟下面这个图片类似：</div>
<div><a href="http://www.bio-info-trainee.com/wp-content/uploads/2016/07/chip-seq-workflow-all-5-steps.jpg"><img class="alignnone size-full wp-image-1759" src="http://www.bio-info-trainee.com/wp-content/uploads/2016/07/chip-seq-workflow-all-5-steps.jpg" alt="chip-seq-workflow-all-5-steps" width="502" height="540" /></a></div>
<div></div>
<div>
<div>QuEST is a statistical software for analysis of ChIP-Seq data with data and analysis results visualization through UCSC Genome Browser.  <a href="http://www-hsc.usc.edu/">http://www-hsc.usc.edu/</a>~valouev/QuEST/QuEST.html</div>
<div>peak calling 阈值的选择： <a href="http://www.nature.com/nprot/journal/v7/n1/fig_tab/nprot.2011.420_F2.html">http://www.nature.com/nprot/journal/v7/n1/fig_tab/nprot.2011.420_F2.html</a></div>
<div>MeDIP-seq and histone modification ChIP-seq analysis  <a href="http://crazyhottommy.blogspot.com/2014/01/medip-seq-and-histone-modification-chip.html">http://crazyhottommy.blogspot.com/2014/01/medip-seq-and-histone-modification-chip.html</a></div>
<div>2011-review-CHIP-seq-high-quaility-data: <a href="http://www.nature.com/ni/journal/v12/n10/full/ni.2117.html?message-global=remove">http://www.nature.com/ni/journal/v12/n10/full/ni.2117.html?message-global=remove</a></div>
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<div>不同处理条件的CHIP-seq的差异peaks分析： <a href="http://www.slideshare.net/thefacultyl/diffreps-automated-chipseq-differential-analysis-package">http://www.slideshare.net/thefacultyl/diffreps-automated-chipseq-differential-analysis-package</a></div>
<div>一个实际的CHIP-seq数据分析例子： <a href="http://www.biologie.ens.fr/">http://www.biologie.ens.fr/</a>~mthomas/other/chip-seq-training/</div>
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<p><span id="more-1736"></span></p>
<div>
<div><a href="http://biow.sb-roscoff.fr/ecole_bioinfo/training_material/chip-seq/documents/presentation_chipseq.pdf">http://biow.sb-roscoff.fr/ecole_bioinfo/training_material/chip-seq/documents/presentation_chipseq.pdf</a></div>
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<div>
<div><a href="http://ecole-bioinfo-aviesan.sb-roscoff.fr/sites/ecole-bioinfo-aviesan.sb-roscoff.fr/files/files/chipseq_CarlHerrmann_Roscoff2015.pdf">http://ecole-bioinfo-aviesan.sb-roscoff.fr/sites/ecole-bioinfo-aviesan.sb-roscoff.fr/files/files/chipseq_CarlHerrmann_Roscoff2015.pdf</a></div>
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<div>
<div><a href="http://ecole-bioinfo-aviesan.sb-roscoff.fr/sites/ecole-bioinfo-aviesan.sb-roscoff.fr/files/files/defrance-ChIP-seq_annotation.pdf">http://ecole-bioinfo-aviesan.sb-roscoff.fr/sites/ecole-bioinfo-aviesan.sb-roscoff.fr/files/files/defrance-ChIP-seq_annotation.pdf</a></div>
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<p>然后下面的各种资料，是针对CHIP-seq流程的各个环境的，还有一些是针对于表观遗传学知识的</p>
<div>
<div>## ppt : <a href="http://159.149.160.51/epigen_milano/epigen_barozzi.pdf">http://159.149.160.51/epigen_milano/epigen_barozzi.pdf</a></div>
<div>## best practise: <a href="http://bioinformatics-core-shared-training.github.io/cruk-bioinf-sschool/">http://bioinformatics-core-shared-training.github.io/cruk-bioinf-sschool/</a></div>
<div>## pipeline : <a href="https://github.com/shenlab-sinai/chip-seq_preprocess">https://github.com/shenlab-sinai/chip-seq_preprocess</a></div>
<div>## <a href="https://sites.google.com/site/anshul...e/projects/idr">https://sites.google.com/site/anshul...e/projects/idr</a>  ## samtools view -b -F 1548 -q 30 chipSampleRep1.bam</div>
<div>## pipeline : <a href="http://daudin.icmb.utexas.edu/wiki/index.php/ChIPseq_prep_and_map">http://daudin.icmb.utexas.edu/wiki/index.php/ChIPseq_prep_and_map</a></div>
<div>## pipeline : <a href="https://github.com/BradyLab/ChipSeq/blob/master/chipseq.sh">https://github.com/BradyLab/ChipSeq/blob/master/chipseq.sh</a></div>
<div>## <a href="https://github.com/crukci-bioinformatics/chipseq-pipeline">https://github.com/crukci-bioinformatics/chipseq-pipeline</a></div>
<div>## <a href="https://github.com/ENCODE-DCC/chip-seq-pipeline">https://github.com/ENCODE-DCC/chip-seq-pipeline</a></div>
<div></div>
<div>## Hands-on introduction to ChIP-seq analysis - VIB Training   <a href="http://www.biologie.ens.fr/">http://www.biologie.ens.fr/</a>~mthomas/other/chip-seq-training/</div>
<div>## video(A Step-by-Step Guide to ChIP-Seq Data Analysis Webinar) : <a href="http://www.abcam.com/webinars/a-step-by-step-guide-to-chip-seq-data-analysis-webinar">http://www.abcam.com/webinars/a-step-by-step-guide-to-chip-seq-data-analysis-webinar</a></div>
<div>## Using ChIP-Seq to identify and/or quantify bound regions (peaks)  <a href="http://barcwiki.wi.mit.edu/wiki/SOPs/chip_seq_peaks">http://barcwiki.wi.mit.edu/wiki/SOPs/chip_seq_peaks</a></div>
<div>## <a href="http://jura.wi.mit.edu/bio/education/hot_topics/ChIPseq/ChIPSeq_HotTopics.pdf">http://jura.wi.mit.edu/bio/education/hot_topics/ChIPseq/ChIPSeq_HotTopics.pdf</a></div>
<div>## <a href="http://pedagogix-tagc.univ-mrs.fr/courses/ASG1/practicals/chip-seq/mapping_tutorial.html">http://pedagogix-tagc.univ-mrs.fr/courses/ASG1/practicals/chip-seq/mapping_tutorial.html</a></div>
<div>## 公开课： <a href="https://www.coursera.org/learn/galaxy-project/lecture/FUzcg/chip-sequence-analysis-with-macs">https://www.coursera.org/learn/galaxy-project/lecture/FUzcg/chip-sequence-analysis-with-macs</a></div>
<div>##ＥＢＩ的教程：<a href="https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course/chip-seq-analysis/chip-seq-practical">https://www.ebi.ac.uk/training/online/course/ebi-next-generation-sequencing-practical-course/chip-seq-analysis/chip-seq-practical</a></div>
<div>## 日语教程：<a href="http://genomejack.net/download/GenomeJackBrowserAppendix/browser_appendix_j/tutorials/chipSeq.html">http://genomejack.net/download/GenomeJackBrowserAppendix/browser_appendix_j/tutorials/chipSeq.html</a></div>
<div>## 台湾教程：<a href="http://lsl.sinica.edu.tw/Services/Class/files/20151118475_2.pdf">http://lsl.sinica.edu.tw/Services/Class/files/20151118475_2.pdf</a> 徐唯哲 Paul Wei-Che HSU</div>
<div>中央研究院 分子生物研究所</div>
<div>研究助技師</div>
<div>## peak finder软件大全： <a href="http://wodaklab.org/nextgen/data/peakfinders.html">http://wodaklab.org/nextgen/data/peakfinders.html</a></div>
<div>## <a href="https://www.encodeproject.org/documents/049704a4-5c58-4631-acf1-4ef152bdb3ef/@@download/attachment/Learning_Chromatin_States_from_ChIP-seq_data.pdf">https://www.encodeproject.org/documents/049704a4-5c58-4631-acf1-4ef152bdb3ef/@@download/attachment/Learning_Chromatin_States_from_ChIP-seq_data.pdf</a></div>
<div>## <a href="https://bioshare.bioinformatics.ucdavis.edu/bioshare/download/47aq5pp5mzza5vb/PDFs/Tuesday_MB_ChIP-Seq_Intro.pdf">https://bioshare.bioinformatics.ucdavis.edu/bioshare/download/47aq5pp5mzza5vb/PDFs/Tuesday_MB_ChIP-Seq_Intro.pdf</a></div>
<div>## paper： Large-Scale Quality Analysis of Published ChIP-seq Data <a href="http://www.g3journal.org/content/4/2/209.full">http://www.g3journal.org/content/4/2/209.full</a></div>
<div>## paper： Chip-seq data analysis: from quality check to motif discovery and more <a href="http://ccg.vital-it.ch/var/sib_april15/cases/landt12/strand_correlation.html">http://ccg.vital-it.ch/var/sib_april15/cases/landt12/strand_correlation.html</a></div>
<div>## Workshop hands on session(RNA-Seq / ChIP-Seq  ) :  <a href="https://hpc.oit.uci.edu/biolinux/handson.docx">https://hpc.oit.uci.edu/biolinux/handson.docx</a></div>
<div>## <a href="http://www.gqinnovationcenter.com/documents/bioinformatics/ChIPseq.pptx">http://www.gqinnovationcenter.com/documents/bioinformatics/ChIPseq.pptx</a></div>
<div>## paper supplement : <a href="http://genome.cshlp.org/content/suppl/2015/10/02/gr.192005.115.DC1/Supplemental_Information.docx">http://genome.cshlp.org/content/suppl/2015/10/02/gr.192005.115.DC1/Supplemental_Information.docx</a></div>
<div><a href="http://www.illumina.com/documents/products/datasheets/datasheet_chip_sequence.pdf">http://www.illumina.com/documents/products/datasheets/datasheet_chip_sequence.pdf</a></div>
<div></div>
<div><a href="http://www.ncbi.nlm.nih.gov/pubmed/22130887">http://www.ncbi.nlm.nih.gov/pubmed/22130887</a> "Analyzing ChIP-seq data: preprocessing, normalization, differential identification, and binding pattern characterization."</div>
<div><a href="http://www.ncbi.nlm.nih.gov/pubmed/22499706">http://www.ncbi.nlm.nih.gov/pubmed/22499706</a> "Normalization, bias correction, and peak calling for ChIP-seq." (stat heavy)</div>
<div><a href="http://www.ncbi.nlm.nih.gov/pubmed/24244136">http://www.ncbi.nlm.nih.gov/pubmed/24244136</a> "Practical guidelines for the comprehensive analysis of ChIP-seq data."</div>
<div><a href="http://www.ncbi.nlm.nih.gov/pubmed/25223782">http://www.ncbi.nlm.nih.gov/pubmed/25223782</a> "Identifying and mitigating bias in next-generation sequencing methods for chromatin biology."</div>
<div>A quick search also turned up this recent paper (which I haven't read) that might be of interest to you</div>
<div></div>
<div><a href="http://www.ncbi.nlm.nih.gov/pubmed/24598259">http://www.ncbi.nlm.nih.gov/pubmed/24598259</a> "Impact of sequencing depth in ChIP-seq experiments."</div>
<div></div>
<div>## figures: <a href="https://github.com/shenlab-sinai/ngsplot">https://github.com/shenlab-sinai/ngsplot</a></div>
<div><a href="https://github.com/daler/metaseq">https://github.com/daler/metaseq</a></div>
<div><a href="http://liulab.dfci.harvard.edu/CEAS/usermanual.html">http://liulab.dfci.harvard.edu/CEAS/usermanual.html</a></div>
<div>还有两个ｗｅｂ－ｔｏｏｌｓ也是可视化</div>
<div></div>
<div></div>
<div>bioconductor系列工具和教程 :</div>
<div><a href="http://faculty.ucr.edu/">http://faculty.ucr.edu/</a>~tgirke/HTML_Presentations/Manuals/Workshop_Dec_6_10_2012/Rchipseq/Rchipseq.pdf</div>
<div><a href="http://bioinformatics-core-shared-training.github.io/cruk-bioinf-sschool/Day4/chipqc_sweave.pdf">http://bioinformatics-core-shared-training.github.io/cruk-bioinf-sschool/Day4/chipqc_sweave.pdf</a></div>
<div><a href="http://bioconductor.org/packages/release/bioc/html/chipseq.html">http://bioconductor.org/packages/release/bioc/html/chipseq.html</a></div>
<div><a href="http://bioconductor.org/help/workflows/chipseqDB/">http://bioconductor.org/help/workflows/chipseqDB/</a></div>
<div><a href="http://bioconductor.org/help/workflows/generegulation/">http://bioconductor.org/help/workflows/generegulation/</a></div>
<div><a href="http://bioconductor.org/help/course-materials/2009/EMBLJune09/Practicals/chipseq/BasicChipSeq.pdf">http://bioconductor.org/help/course-materials/2009/EMBLJune09/Practicals/chipseq/BasicChipSeq.pdf</a></div>
<div></div>
<div>## 公司教程： <a href="http://www.partek.com/Tutorials/microarray/Tiling/ChipSeqTutorial.pdf">http://www.partek.com/Tutorials/microarray/Tiling/ChipSeqTutorial.pdf</a></div>
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