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	<title>生信菜鸟团 &#187; maq</title>
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		<title>很老的比对软件maq</title>
		<link>http://www.bio-info-trainee.com/1613.html</link>
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		<pubDate>Thu, 05 May 2016 12:16:38 +0000</pubDate>
		<dc:creator><![CDATA[ulwvfje]]></dc:creator>
				<category><![CDATA[基础软件]]></category>
		<category><![CDATA[maq]]></category>
		<category><![CDATA[sam]]></category>
		<category><![CDATA[比对]]></category>

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		<description><![CDATA[MAQ在2008年还是蛮火的，但是现在基本都是BWA和bowtie的天下了。 就 &#8230; <a href="http://www.bio-info-trainee.com/1613.html">Continue reading <span class="meta-nav">&#8594;</span></a>]]></description>
				<content:encoded><![CDATA[<div>MAQ在2008年还是蛮火的，但是现在基本都是BWA和bowtie的天下了。</div>
<div>就当怀念一下它吧，给它写一个教程！</div>
<div><span style="color: #ff0000;">软件下载：</span></div>
<div>官网直接找到：<a href="http://maq.sourceforge.net/">http://maq.sourceforge.net/</a></div>
<div>我是linux系统，用wget下载：wget <a href="https://sourceforge.net/projects/maq/files/maq/0.7.1/maq-0.7.1.tar.bz2">https://sourceforge.net/projects/maq/files/maq/0.7.1/maq-0.7.1.tar.bz2</a></div>
<div>解压，很容易观察到是C++源码，所以用源码安装三部曲来安装</div>
<blockquote>
<div>tar jxvf software.tar.bz2</div>
<div>cd software</div>
<div>./configure --prefix=$path</div>
<div>make</div>
<div>make test</div>
</blockquote>
<div>安装之后把该软件添加到环境变量！</div>
<div>输入数据：</div>
<div>这里选择两个网络上的测试数据:</div>
<div>如果是真想用这个软件的话，需要参考基因组和测序数据，这个链接貌似已经年久失修啦~！</div>
<blockquote>
<div><code>wget <a href="http://biocluster.ucr.edu/~tbackman/genome.fasta">http://biocluster.ucr.edu/~tbackman/genome.fasta</a> </code></div>
<div><code># download a test reference genome (TAIR9 Chromosome 1)<br />
wget </code><code><a href="http://biocluster.ucr.edu/~tbackman/query.fastq">http://biocluster.ucr.edu/~tbackman/query.fastq</a></code><code> </code></div>
<div><code># download some test Illumina reads from Arabidopsis</code></div>
</blockquote>
<div>
<div></div>
</div>
<p><span style="color: #ff0000;">运行命令：</span></p>
<blockquote>
<div><code>maq # inspect command line options</code><br />
<code>maq fasta2bfa genome.fasta genome.bfa</code><br />
<code>   # create binary of reference genome</code><br />
<code>maq fastq2bfq query.fastq readBinary.bfq</code><br />
<code>   # create a binary of dataset</code><br />
<code>maq match out.map genome.bfa readBinary.bfq</code><br />
<code># align query to genome and store output</code></div>
</blockquote>
<p><span style="color: #ff0000;">结果解读：</span></p>
<div>我在想，这个MAQ软件发明之前，好像还没有SAM文件格式的定义，那么它的结果<code>out.map肯定不是sam格式的。</code></div>
<div><code>哈哈，这个软件我无法安装，换了好几系统也没成功，如果是太老了，很多库文件却是。</code></div>
<div><code>我也懒得去解决了。</code></div>
<div><code>这种报错，对我这样的非计算机专业来说，简直是天书！</code></div>
<div><code><a href="http://www.bio-info-trainee.com/wp-content/uploads/2016/05/14.png"><img class="alignnone size-full wp-image-1614" src="http://www.bio-info-trainee.com/wp-content/uploads/2016/05/14.png" alt="1" width="693" height="332" /></a></code></div>
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