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	<title>生信菜鸟团 &#187; 个体</title>
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		<title>WES（四）不同个体的比较</title>
		<link>http://www.bio-info-trainee.com/1138.html</link>
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		<pubDate>Sun, 01 Nov 2015 10:05:25 +0000</pubDate>
		<dc:creator><![CDATA[ulwvfje]]></dc:creator>
				<category><![CDATA[全外显子组软件]]></category>
		<category><![CDATA[个体]]></category>

		<guid isPermaLink="false">http://www.bio-info-trainee.com/?p=1138</guid>
		<description><![CDATA[3-4-5分别就是孩子、父亲、母亲 我对每个个体取他们的四种软件的公共snp来进 &#8230; <a href="http://www.bio-info-trainee.com/1138.html">Continue reading <span class="meta-nav">&#8594;</span></a>]]></description>
				<content:encoded><![CDATA[<p>3-4-5分别就是孩子、父亲、母亲</p>
<p>我对每个个体取他们的四种软件的公共snp来进行分析，并且只分析基因型，看看是否符合孟德尔遗传定律</p>
<p><a href="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0014.png"><img class="alignnone  wp-image-1144" src="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0014.png" alt="image001" width="653" height="555" /></a></p>
<p>结果如下：</p>
<p><a href="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0024.png"><img class="alignnone size-full wp-image-1145" src="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0024.png" alt="image002" width="522" height="203" /></a></p>
<p>粗略看起来好像很少不符合孟德尔遗传定律耶</p>
<p>然后我写了程序计算</p>
<p><a href="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0034.png"><img class="alignnone size-full wp-image-1146" src="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0034.png" alt="image003" width="546" height="158" /></a></p>
<p>总共127138个可以计算的位点，共有18063个位点不符合孟德尔遗传定律，而且它们在染色体的分布情况如下</p>
<p>我检查了一下，不符合的原因，发现我把</p>
<p>chr1 100617887 C T:DP4=0,0,36,3 T:1/1:40 T:1/1:0,40:40 miss T:DP4=0,0,49,9 T:1/1:59 T:1/1:0,58:59 miss T:DP4=0,0,43,8 T:1/1:53 T:1/1:0,53:53 T:1/1:50</p>
<p>计算成了chr1 100617887 C 0/0 0/0 1/1 所以认为不符合，因为我认为只有四个软件都认为是snp的我才当作是snp的基因型，否则都是0/0</p>
<p>那么我就改写了程序，全部用gatk结果来计算。这次可以计算的snp有个176036，不符合的有20309，而且我看了不符合的snp的染色体分布，Y染色体有点异常</p>
<p><a href="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0044.png"><img class="alignnone size-full wp-image-1147" src="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0044.png" alt="image004" width="784" height="85" /></a> <a href="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0052.png"><img class="alignnone size-full wp-image-1148" src="http://www.bio-info-trainee.com/wp-content/uploads/2015/11/image0052.png" alt="image005" width="786" height="84" /></a></p>
<p>但是很失败，没什么发现！</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
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